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. 2019 Oct 23;6:110. doi: 10.3389/fmolb.2019.00110

Figure 10.

Figure 10

Cross-linking analysis of hASCT2. In (A), immunoblot analysis of protein extracted from HeLa cells treated with 0.75% formaldehyde as described in Materials and Methods. Samples (50 μg total lysate) were subjected to SDS-PAGE and immunoblot with anti-hASCT2 (1:1,000); (–), control without formaldehyde; (+), sample treated with formaldehyde. In (B), immunoblot analysis of purified hASCT2 treated with 1 or 4% formaldehyde as described in Materials and Methods. Samples were subjected to SDS-PAGE and immunoblot with anti-hASCT2 (1:1,000).