Table 3.
List of target genes and their primers evaluated in the rabbits.
| S/N | Gene Name | Primers type | Primer Sequence (5′ to 3′) | Primers Length (bp) | Product size (bp) | Accession No |
|---|---|---|---|---|---|---|
| 1 | Oviductal glycoprotein 1 (OVGP1) | F | TTCCTTGCCTGAGACCAGTT | 20 | 121 | NM_001105687.1 |
| R | CTCTCTCTCGGGGTGGTCATC | 21 | ||||
| 2 | Uncoupling protein 1 (UCP1) | F | CCTAACAACTGGAGGCGTGG | 20 | 177 | NM_001171077.1 |
| R | GGAGTTGTCCCTTTCCACAGA | 21 | ||||
| 3 | Oxidative stress responsive 1 (OXSR1) | F | GTTTGCGTAAAGGTGGGTGG | 20 | 120 | XM_008252717.2 |
| R | AGCAGGCACACACATCACTG | 20 | ||||
| 4 | Brain and reproductive organ-expressed (TNFRSF1A modulator) (BRE) | F | TCAGTCACTTTGGCACAGGT | 20 | 120 | XM_002709945.3 |
| R | GGAAAAACAGGGGCAGGTCA | 20 | ||||
| 5 | Oryctolagus cuniculus superoxide dismutase 1 (SOD1) | F | GGTGGTCAAGGGACGCATAA | 20 | 205 | NM_001082627.2 |
| R | CACATCAGCCACACCATTGC | 20 | ||||
| 6 | Oryctolagus cuniculus glutathione peroxidase 1 (GPX1) | F | TTTGGGCATCAGGAGAACGC | 20 | 211 | NM_001085444.1 |
| R | TGATGAACTTGGGGTCGGTC | 20 | ||||
| 7 | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | F | TCGGAGTGAACGGATTTGGC | 20 | 181 | NM_001082253.1 |
| R | TTCCCGTTCTCAGCCTTGAC | 20 |