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. 2019 Sep 17;18:388–399. doi: 10.1016/j.omtn.2019.09.005

Figure 6.

Figure 6

MALAT1 Acted as a ceRNA against miR-199a, and ZHX1 Was a Target of miR-199a in GBM Cells

(A) Schematic representation of the putative miR-199a binding sites in MALAT1. (B) Schematic representation of the putative miR-199a binding sites in ZHX1. (C) Dual-luciferase reporter assay in cells co-transfected with the WT or mutant reporter plasmid and miR-199a. (D) RIP assay using anti-Ago2, compared to anti-IgG in cells. (E) Subcellular fractionation and FISH staining confirmed the expression of MALAT1 in cytoplasm. (F) Dual-luciferase reporter assay in ZXH1-WT and ZHX1-mut cells transfected with different vectors. (G) Luciferase reporter assay in HEK293T cells co-transfected with the Luc-ZHX1-3′ UTR and the indicated miRNAs. *p < 0.05.