FIG 2.

Inflammatory mediators in nasal secretions after EHV-1 challenge infection. Three groups of horses (n = 8/group) were challenged intranasally with neuropathogenic EHV-1 Ab4 (arrows). Control/Ab4 horses were susceptible at the time of challenge, while horses in the previously infected Ab4ΔORF2/Ab4 and Ab4/Ab4 groups were protected from clinical disease. Cytokines and chemokines were quantified in the nasal secretions of all horses using fluorescent bead-based multiplex assays detecting sCD14 (A), IFN-α (B), IFN-γ (C), IL-10 (D), CCL2 (E), CCL3 (F), CCL11 (G), and CCL5 (H). Mean and standard errors are shown for each group. Significant differences between groups are marked as a (control/Ab4 versus Ab4ΔORF2/Ab4), b (control/Ab4 versus Ab4/Ab4), and c (Ab4ΔORF2/Ab4 versus Ab4/Ab4).