TABLE 1.

Protection of horses based on body temperature, nasal shedding of infectious virus, and cell-associated viremia after challenge infection with EHV-1^a

Horse information			Maximum feverb		Maximum sheddingc		Maximum viremiad
EHV-1 protection status	ID	Group	°C	Hours p.i.	PFU/ml	Day p.i.	CTe	Day p.i.
Fully protected	1	Ab4ORF2/Ab4	No fever	NA	No shedding	NA	No viremia	NA
	8	Ab4ORF2/Ab4	No fever	NA	No shedding	NA	No viremia	NA
	14	Ab4ORF2/Ab4	No fever	NA	No shedding	NA	No viremia	NA
	18	Ab4ORF2/Ab4	No fever	NA	No shedding	NA	No viremia	NA
	23	Ab4ORF2/Ab4	No fever	NA	No shedding	NA	No viremia	NA
	6	Ab4/Ab4	No fever	NA	No shedding	NA	No viremia	NA
	11	Ab4/Ab4	No fever	NA	No shedding	NA	No viremia	NA
	24	Ab4/Ab4	No fever	NA	No shedding	NA	No viremia	NA
Partially protected	2	Ab4ORF2/Ab4	No fever	NA	No shedding	NA	36.96	8
	12	Ab4ORF2/Ab4	38.9	NA	No shedding	NA	No viremia	NA
	17	Ab4ORF2/Ab4	No fever	NA	10	1	No viremia	NA
	4	Ab4/Ab4	No fever	NA	30	1	35.82	6
	10	Ab4/Ab4	No fever	NA	No shedding	NA	36.96	7
	13	Ab4/Ab4	No fever	NA	140	2	No viremia	NA
	16	Ab4/Ab4	No fever	NA	2,900	1	No viremia	NA
	21	Ab4/Ab4	No fever	NA	No shedding	NA	30.20	8
Susceptible	3	Control/Ab4	41.2	36	19,500	3	31.25	6
	5	Control/Ab4	41.4	48	700,000	2	30.43	4
	7	Control/Ab4	41.1	60	29,500	2	30.13	6
	9	Control/Ab4	40.9	48	8,000	2	35.43	8
	15	Control/Ab4	41.6	48	240,000	2	33.71	5
	19	Control/Ab4	41.3	36	7,550	3	32.59	6
	20	Control/Ab4	41.4	48	52,500	2	31.32	7
	22	Control/Ab4	41.2	36	28,000	2	32.89	8

^aNA, not applicable.

^bBody temperature >38.5°C considered a fever.

^cVirus isolation in nasal secretion (PFU/ml).

^dDetection by real-time PCR of EHV-1 gB in PBMC.

^eC_T, cycle threshold.