FIG 1.

Characterization of the recombinant MVA-vpx genome. (A) PCR analysis of DNA from the indicated viruses. In MVA-vpx-249, vpx expression is controlled by the mH5 (early/late) promoter, and in MVA-vpx-253 it is controlled by the early 13.5L promoter. The genes were inserted into the region of deletion III of MVA. (B) Primers used for the analysis of the deletion III region and the K1L gene. Sequence information is given in the Materials and Methods. Schematic representation of the recombinant MVA. P, promoter. Analysis of the deletion III region confirmed the insertion of sequences into this site. Deletion IV and the C7L gene were not altered, and the K1L gene was deleted.