FIG 3.

The late B5 protein is expressed in MVA-vpx-infected DCs and correlates with Vpx expression and a decrease in SAMHD1 protein. Human DCs were infected with VACV WR, MVA, MVA-vpx-249, and MVA-vpx-253 at an MOI of 3. Cells were harvested at 6 h after infection, proteins were separated by SDS-PAGE, and Western blot analysis was performed with an antibody directed against B5, a late-expressed VACV protein. In addition, Vpx and SAMHD1 expression were analyzed with specific antibodies. Equal loading was confirmed by detection of β-actin. Experiments were performed three times, and representative results are shown.