FIG 4.

Insertion of vpx into the MVA genome increased viral DNA replication in DCs. Viral DNA replication was analyzed by quantitative real-time PCR of the highly conserved rpo18 gene. The template DNA was isolated from infected human DCs (MOI = 3) 24 h after infection, and 37.5 μg template DNA was used for each reaction. The amount of DNA measured in samples from WR-infected cells was set to 100%. Average values obtained from four experiments are given with standard deviations, and the P values are indicated. ***, P = 0.002 between MVA and MVA-vpx-249 infection; ****, P < 0.0001 between MVA and MVA-vpx-253 infection. P values were calculated with GraphPad Prism 7.04 software as unpaired two-tailed t tests.