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Expression of Vpx by MVA does not influence apoptosis induction. The induction of apoptosis was measured using flow cytometry as caspase 3 activation. Human DC cells were infected at an MOI of 3 with the four viruses and then harvested at the indicated time points, permeabilized, and stained with an antibody directed against active caspase 3. Cells treated with 1 μM staurosporine (Stauro) were used as a positive control. The percentages of active caspase 3-positive cells are indicated.
