FIG 1.

Generation of Ifnl2^−/− Ifnl3^−/− mice. (A) Diagram depicting the guide RNA (bold purple arrow) used to target Ifnl3 for insertion of a construct with a left homology arm (HA), a single T2A sequence, a tdTomato-coding sequence with a polyadenylation signal, and a right HA (blue) by the use of CRISPR/Cas9. Locations of primers used to verify insertion are shown by small arrows over the 5′ and 3′ junctions. (B) PCR amplification of 5′ and 3′ insertion junctions using primers corresponding to the small arrows described in the panel A legend. (C) Schematic of Ifnl2 and Ifnl3 loci showing 7.2-kb deletion and insertion site of tdTomato with portions of genomic sequence (blue), nonspecific inserted sequences (black), and the construct sequence (red) at junctions.