FIG 6.

The NTD of STAT2 interacts with nsp11. (A) Schematic illustration of truncation plasmids STAT2: D1, D2, and D3. The numbers above the lines indicate amino acid positions in STAT2. N, N-terminal domain; CCD, coiled-coil domain; DBD, DNA binding domain; L, linker domain; SH2,: Src homology 2 domain; TAD, transactivation domain. (B) IP of nsp11-NTD coprecipitates STAT2, STAT2-D1, and STAT2-D2, but not STAT2-D3. HEK293 cells were transfected with YFP-nsp11-NTD and Myc-STAT2 or STAT2 truncate plasmids for 36 h. IP with YFP antibody, followed by WB with cMyc and YFP antibodies, was done. EV was included as a control. WB of the input was done.