FIG 6.

Inhibitory activity of GPI-anchored peptides during cell-cell transmission of HIV-1 pseudoviruses. (A) Single-cycle infections of TZM-bl cells by a panel of cell-free HIV-1 pseudoviruses are dependent on DEAE-dextran. (B) Single-cycle infections of TZM-bl cells by the cell-associated HIV-1 pseudoviruses are independent of DEAE-dextran. (C) Inhibition of GPI-anchored peptides during cell-cell transmission mediated by the HIV-1 pseudoviruses. Transduced TZM-bl cells were used as a target and cocultured for 36 h with donor HEK293T cells that were preinfected by pseudoviruses, and percent infection of TZM-bl cells was monitored by quantifying the production of the reporter luciferase. Error bars represent the means ± SEM from three independent experiments with triplicate samples.