TABLE 1.
Inhibitory activity of GPI-2P23 against divergent HIV-1 and T20-resistant mutantsa
| HIV-1 isolate | Subtype | Tropism | Mean % infection ± SEM |
||
|---|---|---|---|---|---|
| GPI-4B10 | GPI-C34 | GPI-2P23 | |||
| Replication-competent viruses | |||||
| JR-CSF | B | CCR5 | 92 ± 3 | 0 | 0 |
| THRO.c/2626 | B | CCR5 | 91 ± 3 | 0 | 0 |
| CH077.t/2627 | B | CCR5 | 83 ± 3 | 0 | 0 |
| NL4-3 | B | CXCR4 | 97 ± 3 | 0 | 0 |
| LAI.2 | B | CXCR4 | 84 ± 3 | 0 | 0 |
| SG3.1 | B | CXCR4 | 92 ± 3 | 0 | 0 |
| 89.6 | B | R5X4 | 91 ± 3 | 0 | 0 |
| Mj4 | C | CCR5 | 94 ± 3 | 0 | 0 |
| “Global panel” pseudovirus | |||||
| 398-F1_F6_20 | A | CCR5 | 91 ± 3 | 0 | 0 |
| TRO.11 | B | CCR5 | 89 ± 3 | 0 | 0 |
| X2278_C2_B6 | B | CCR5 | 98 ± 3 | 0 | 0 |
| CE1176_A3 | C | CCR5 | 87 ± 3 | 0 | 0 |
| CE703010217_B6 | C | CCR5 | 86 ± 2 | 0 | 0 |
| HIV_25710-2.43 | C | CCR5 | 84 ± 2 | 0 | 0 |
| X1632-S2-B10 | G | CCR5 | 99 ± 4 | 0 | 0 |
| 246_F3_C10_2 | A/C | CCR5 | 91 ± 4 | 0 | 0 |
| CNE8 | A/E | CCR5 | 89 ± 3 | 0 | 0 |
| CNE55 | A/E | CCR5 | 91 ± 2 | 0 | 0 |
| CH119.10 | B/C | CCR5 | 97 ± 5 | 0 | 0 |
| BJOX002000.03 | B/C | CCR5 | 99 ± 4 | 0 | 0 |
| T20-resistant isolates | |||||
| NL4-3WT | B | CXCR4 | 98 ± 3 | 0 | 0 |
| NL4-3I37T | B | CXCR4 | 96 ± 2 | 0 | 0 |
| NL4-3V38A | B | CXCR4 | 93 ± 2 | 0 | 0 |
| NL4-3V38M | B | CXCR4 | 97 ± 2 | 0 | 0 |
| NL4-3Q40H | B | CXCR4 | 97 ± 2 | 0 | 0 |
| NL4-3N43K | B | CXCR4 | 98 ± 3 | 0 | 0 |
| NL4-3D36S/V38M | B | CXCR4 | 98 ± 3 | 0 | 0 |
| NL4-3I37T/N43K | B | CXCR4 | 101 ± 5 | 1 | 0 |
| NL4-3V38A/N42T | B | CXCR4 | 103 ± 3 | 1 | 0 |
a
The assay was performed in triplicate and repeated 3 times. The data for all 3 assays are expressed as means ± standard errors of means. NL4-3WT, wild-type NL4-3.