TABLE 2.
Inhibitory activity of GPI-2P23 against HIV-1 Env-mediated cell-cell fusiona
| HIV-1 Env | Subtype | Tropism | Mean % cell-cell fusion ± SEM |
||
|---|---|---|---|---|---|
| GPI-4B10 | GPI-C34 | GPI-2P23 | |||
| Primary Envs | |||||
| 92RW020 | A | CCR5 | 112 ± 5 | 0 | 0 |
| JRFL | B | CCR5 | 107 ± 4 | 0 | 0 |
| R3A | B | R5/X4 | 99 ± 2 | 0 | 0 |
| CNE11 | B′ | R5/X4 | 92 ± 8 | 0 | 0 |
| CAP210.2.00.E8 | C | CCR5 | 100 ± 5 | 0 | 0 |
| CNE8 | A/E | CCR5 | 89 ± 6 | 0 | 0 |
| CH70.1 | B/C | R5/X4 | 102 ± 1 | 0 | 0 |
| Envs of T20-resistant mutants | |||||
| NL4-3WT | B | CXCR4 | 92 ± 3 | 0 | 0 |
| NL4-3I37T | B | CXCR4 | 98 ± 3 | 5 ± 1 | 0 |
| NL4-3V38A | B | CXCR4 | 99 ± 3 | 5 ± 1 | 0 |
| NL4-3V38M | B | CXCR4 | 93 ± 3 | 4 ± 1 | 0 |
| NL4-3Q40H | B | CXCR4 | 89 ± 3 | 8 ± 1 | 0 |
| NL4-3N43K | B | CXCR4 | 92 ± 2 | 3 ± 1 | 0 |
| NL4-3D36S/V38M | B | CXCR4 | 101 ± 6 | 0 | 0 |
| NL4-3I37T/N43K | B | CXCR4 | 99 ± 2 | 26 ± 3 | 0 |
| NL4-3V38A/N42T | B | CXCR4 | 98 ± 3 | 16 ± 3 | 0 |
a
The assay was performed in triplicate and repeated 3 times. The data for all 3 assays are expressed as means ± standard errors of means.