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. 2019 Oct 29;93(22):e01273-19. doi: 10.1128/JVI.01273-19

FIG 4.

FIG 4

HCV NS3 and NS3/4A inhibit NHEJ repair. (A and B) NHEJ assay. The overall and precise NHEJ efficiencies in HCVR and in 293T or Huh7 cells expressing NS3 or NS3/4A protein were measured by luciferase assay. The luminescence value of firefly luciferase was normalized against the internal EGFP fluorescence control (A) or Renilla luminescence control (B). Statistical analysis is shown (n = 3). P values define significant differences between the NHEJ efficiencies of the Huh7 and HCVR cells or between those of the negative control (NC) and NS3- or NS3/4A-expressing cells. (C) HR assay. The HR efficiency in 293T cells expressing NS3 or NS3/4A protein was calculated by normalization of the percentage of EGFP-positive cells to the luminescence value of the firefly luciferase control. Statistical analysis is shown (n = 3). P values define significant differences between those of the negative control (NC) and NS3- or NS3/4A-expressing cells. *, P < 0.05; ***, P < 0.001.