FIG 7.

The level of WRN protein is regulated by proteasome and HCV NS3 protease activity. (A) WRN level is regulated by both proteasome-dependent and -independent degradation pathways. TR-NS3/4A cells induced by Dox for 48 h were treated with the proteasome inhibitor MG132 20 min before the CHX treatment or treated with CHX or MG132 alone, as indicated. The cells were harvested 8 h after the CHX treatment for Western blot analysis. Statistical analysis is shown (n = 3). *, P < 0.05; **, P < 0.01. (B) HCV NS3/4A reduces the stability of WRN. After Dox induction, TR-NS3/4A and TR-NS3pd/4A cells were treated with CHX and harvested at the indicated time points for Western blot analysis. Plot shows the statistical analysis (n = 3). ***, P < 0.001.