FIG 3.

MEV NS1-induced cell death. (A and B) Effects of MEV NS1, VP1, and VP2 on viability of F81 cells (A) and HEK293T cells (B). NS1-, VP1-, VP2- or vector-transfected cells were harvested at the indicated times postinfection for determination of cell viability using the MTT assay. (C and D) The release of LDH was used to assess the cell lysis of transfected F81 cells (C) and HEK293T cells (D) at different time points posttransfection. Z-DEVD-FMK and cisplatin were used as the negative and positive controls for apoptosis, respectively. DMSO was used as a vehicle control. For all assays, data are means ± SEMs, and results from a representative experiment of three independent experiments are shown. *, P < 0.05, and **, P < 0.01, compared with control groups. hpt, hour posttransfection.