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MEV NS2 did not induce apoptosis. HEK293T cells were transfected with NS2-expressing plasmid or empty vector. At 24, 48, and 72 h posttransfection, the cell cycle (A) and apoptotic cells (B) were analyzed by flow cytometry. The histograms show results from representative experiments. The bar charts show the percentages of the cells in G1 and G2 phases (A) and the rates of apoptotic cells (B), respectively.
