TABLE 1.
Primer sequences used for amplification of MEV genes
| Primer name | Sequence (5′–3′) | Positionsa |
|---|---|---|
| MEV NS1-F/R | CGC CTG CAG ATG TCT GGC AAC CAG TAT A | 155–2161 |
| CGG GGA TCC TTA ATC CAA GTC GTC TCGAA | ||
| MEV VP2-F/R | GGA TCC ACC ATG AGT GAT GGA G | 2669–4423 |
| CCG CTC GAG TCA ATA TAA TTT TCT AGG | ||
| NS1 1-337 F/R | AAG GAT CCG CCA CCA TGT CTG GCA ACC | 155–1165 |
| GGA CGC GTC GAC TTA TCT TGC TAA AGT CA | ||
| NS1 1-556 F/R | AAG GAT CCG CCA CCA TGT CTG GCA ACC | 155–1823 |
| CAC GCG TCG ACT TAT CCC CAA TGA TGT GT | ||
| NS1 338-556 F/R | GGA TCC GCC ACC ATG ACA AAA ACA GCA TTT | 1166–1823 |
| CAC GCG TCG ACT TAT CCC CAA TGA TGT GT | ||
| NS1 338-668 F/R | GGA TCC GCC ACC ATG ACA AAA ACA GCA TTT | 1166–2161 |
| GCC GCG TCG ACT TAA TCC AAG TCG TCT CG | ||
| NS1 557-668 F/R | GGA TCC GCC ACC ATG AAA GTA CCA GAA TGG G | 1823–2161 |
| GCC GCG TCG ACT TAA TCC AAG TCG TCT CG |
a
Primer positions refer to the nucleotide sequence of strain MEVB (GenBank accession number FJ592174). Boldface and underlined sequences represent restriction sites.