KEY RESOURCES TABLE
| REAGENT or RESOURCE Antibodies | SOURCE | IDENTIFIER |
|---|---|---|
| Rat anti-mouse CD90.1 (Thy1.1) clone: HIS51, APC-eFluor780 | ThermoFisher | Cat#47-0900-82 |
| Rat anti-mouse CD90.2 (Thy1.2) clone: 53-2.1, APC-eFluor780 | ThermoFisher | Cat#47-0902-82 |
| Rat anti-mouse CD105, clone: MJ7/18, biotin | ThermoFisher | Cat#13-1051-85 |
| Rat anti-mouse CD200, clone: 0X-90, BV605 | BD Biosciences | Cat#745255 |
| Rat anti-mouse CD45, clone: 30-F11, PE-Cy5 | Biolegend | Cat#103110 |
| Rat anti-mouse TER119, clone: TER119, PE-Cy5 | ThermoFisher | Cat#15-5921-81; RRID: AB_468809 |
| Rat anti-mouse TIE2 (CD202B), clone: TEK4 | ThermoFisher | Cat#14-5987-81; RRID: AB_466847 |
| Rat anti-mouse ITGAV CD51, clone: RMV-7, PE | BD Biosciences | Cat#551187; RRID: AB_394088 |
| Goat anti-rat IgG secondary antibody, Qdot 605 | ThermoFisher | Cat#Q-11601MP; RRID: AB_2556476 |
| Alexa Fluor 680 Fluorophore Antibody Labeling Kit | ThermoFisher | Cat#A20188 |
| Mouse anti-mouse anti-PGP9.5, clone: 13C4 | Abcam | Cat#Ab8189; RRID: AB_306343 |
| Rabbit anti-mouse myelin PLP | Abcam | Cat#Ab28486; RRID: AB_776593 |
| Rabbit anti-mouse S100 | Abcam | Cat#Ab868; RRID: AB_306716 |
| Rabbit anti-mouse CD34 | Abcam | Cat#Ab81289; RRID: AB_1640331 |
| Rabbit anti-mouse Collagen 1 | Abcam | Cat#Ab34710; RRID: AB_731684 |
| Rabbit anti-mouse Runx2 | Abcam | Cat#Ab23981; RRID: AB_777785 |
| Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor 488 | ThermoFisher | Cat#A28175; RRID: AB_2536161 |
| Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 | ThermoFisher | Cat#A-21245; RRID: AB_2535813 |
| Isolectin GS-IB4 From Griffonia simplicifolia, Alexa Fluor 647 Conjugate | ThermoFisher | Cat#I32450 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Tamoxifen | Sigma | Cat#T5648 |
| Corn Oil | Sigma | Cat#C8267 |
| Alizarin Red S | Sigma | Cat#H7904 |
| Alcian Blue | Sigma | Cat#83157 |
| Ammonium Hydroxide | Fisher Chemical | Cat#A669-500 |
| Hematoxylin | Sigma | Cat#H3136 |
| Brilliant Crocein | ChemCruz | CAS# 5413-75-2 |
| Acid Fuchsin | Sigma | Cat#F8129 |
| Ferric Chloride | PolyScientific | CAS#10025-77-1 |
| Acetic Acid | EMD Millipore | Cat#AX0073 |
| Phosphotungstic Acid | Sigma | Cat#P4006 |
| Saffron | Sigma | Cat#S8381 |
| Nuclear Fast Red solution | Sigma | CAS# 6409-77-4 |
| Eosin Y solution, alcoholic | Sigma | Cat#HT1101128 |
| Ethanol | Gold Shield | Cat#64-17-5 |
| Permount | Fisher Chemicals | Cat#SP15 |
| Triton X-100 | Sigma | Cat#X100 |
| DAPI (4’, 6-Diamido-2-Phenylindole, Dihydrochloride) | ThermoFisher | Cat#D1306 |
| Millicell Cell Culture Inserts | EMD Millipore | Cat#MCHT24H48 |
| Recombinant Murine PDGF-AA | PeproTech | Cat#315-17 |
| Parathyroid Hormone (PTH) (1-34), Human | GenScript | Cas# 52232-67-4 |
| Recombinant Human Oncostatin M (OSM) Protein | R&D Systems | Cat#295-OM/CF |
| Corning Matrigel Membrane Matrix | ThermoFisher | Ca#356234 |
| Fluoromount-G | SouthernBiotech | Cat#0100-01 |
| Critical Commercial Assays | ||
| Click-iT EdU Alexa Fluor 488 Imaging Kit | ThermoFisher | Cat#C10337 |
| StemPro Osteogenesis Differentiation Kit | ThermoFisher | Cat#A1007201 |
| SuperScript VILO cDNA Synthesis Kit | ThermoFisher | Cat#11755-050 |
| TaqMan PreAmp Master Mix | ThermoFisher | Cat#4391128 |
| PowerUp SYBR Green | Applied Biosystems | Cat#A25741 |
| Experimental Models: Organisms/Strains | ||
| C57BL/6J | The Jackson Laboratory | Cat#000664 |
| B6.Cg-Tg(Plp1-cre/ERT)3Pop/J | The Jackson Laboratory | Cat#005975 |
| B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J | The Jackson Laboratory | Cat#007914 |
| NOD.CB17-Prkdcscid/J | The Jackson Laboratory | Cat# 001303 |
| Oligonucleotides | ||
| See Table S1 | ||
| Software and Algorithms | ||
| NRecon | Bruker | N/A |
| CTAn | Bruker | N/A |
| CTVol | Bruker | N/A |
| ImageJ | National Institutes of Health | RRID:SCR_002285 |
| Adobe Photoshop CC | Adobe | RRID:SCR_014199 |
| Adobe Illustrator CC | Adobe | RRID:SCR_010279 |
| Prism 5 | GraphPad | RRID:SCR 002798 |