Table 1.
Composition of glycosphingolipids in the liver of chow-fed and LCKD-fed mice.
| Strain | GSL | Relative level |
P-value | ||
|---|---|---|---|---|---|
| chow | LCKD | Ratio (LCKD/chow) | |||
| ob/ob (n = 3) | GM3-Ac | 2167 ± 259 | 13098 ± 2981 | 6.04 | 0.0232* |
| GM3-Gc | 6993 ± 1027 | 25475 ± 3715 | 3.64 | 0.0011** | |
| GM2-Gc | 110610 ± 11842 | 355805 ± 56026 | 3.22 | 0.0018** | |
| GM1-Ac | 536 ± 121 | 2211 ± 298 | 4.13 | 0.0008*** | |
| GM1-Gc | 1066 ± 192 | 4189 ± 580 | 3.93 | 0.0009*** | |
| GD1a | 3624 ± 2956 | 5762 ± 779 | 1.59 | 0.2926 | |
| Total | 124997 ± 11586 | 406540 ± 60811 | 3.25 | 0.0014** | |
| C57BL/6J (n = 4) | GM3-Ac | 14425 ± 885 | 29321 ± 6231 | 2.03 | 0.0163* |
| GM3-Gc | 26669 ± 2621 | 35370 ± 6603 | 1.33 | 0.0498* | |
| GM2-Gc | 539891 ± 36795 | 429724 ± 95956 | 0.80 | 0.0757 | |
| GM1-Ac | 1386 ± 260 | 3035 ± 521 | 2.19 | 0.0013** | |
| GM1-Gc | 3924 ± 438 | 6352 ± 1319 | 1.62 | 0.0129* | |
| GD1a | 8751 ± 1401 | 10200 ± 2346 | 1.17 | 0.3296 | |
| Total | 595046 ± 41119 | 514003 ± 112597 | 0.86 | 0.2251 | |
Relative levels of glycosphingolipids in the liver were calculated based on the peak area (μV∙sec) of each glycosphingolipid detected in HPLC analysis, as described in the “Experimental design, materials, and methods” section. Values include previously reported data for ob/ob mice [1]. Mean ± S.D.; *P < 0.05, **P < 0.01, ***P < 0.001, chow-fed vs. LCKD-fed. Abbreviations: Ac, N-acetylneuraminic acid; Gc, N-glycolylneuraminic acid; GSL, glycosphingolipid; ob/ob, B6.Cg-Lepob/J.