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. 2019 Oct 2;27:104604. doi: 10.1016/j.dib.2019.104604

Specifications Table

Subject Nutritional science, metabolism
Specific subject area Low-carbohydrate ketogenic diet (LCKD), glycoprotein, glycosphingolipid
Type of data Graph and Table
How data were acquired Enzyme-linked lectin/immunosorbent assays, real-time PCR, HPLC
Data format Raw and analyzed
Parameters for data collection Mice fed a LCKD
Description of data collection Five-week-old female C57BL/6J and B6.Cg-Lepob/J mice were raised on either regular chow or a LCKD (F3666) for 7 weeks, after which tissue samples were collected. Glycoproteins were analyzed by enzyme-linked lectin/immunosorbent assays. Gene expression was analyzed by Agilent Expression Microarray and real-time PCR using SYBR Green I dye as the intercalator. Glycosphingolipids were analyzed by HPLC using a fluorescent labeling method [1].
Data source location Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, Tsukuba, Japan
Data accessibility With the article (Supplemental data) or a public repository
Repository name: NCBI Gene Expression Omnibus (GEO)
Data identification number: GSE115342
Direct URL to data: http://www.ncbi.nlm.nih.gov/geo/
Related research article T. Okuda. A low-carbohydrate ketogenic diet promotes ganglioside synthesis via the transcriptional regulation of ganglioside metabolism-related genes. Scientific Reports. 9 (2019) 7627. https://doi.org/10.1038/s41598-019-43952-7 [1].