Subject | Nutritional science, metabolism |
Specific subject area | Low-carbohydrate ketogenic diet (LCKD), glycoprotein, glycosphingolipid |
Type of data | Graph and Table |
How data were acquired | Enzyme-linked lectin/immunosorbent assays, real-time PCR, HPLC |
Data format | Raw and analyzed |
Parameters for data collection | Mice fed a LCKD |
Description of data collection | Five-week-old female C57BL/6J and B6.Cg-Lepob/J mice were raised on either regular chow or a LCKD (F3666) for 7 weeks, after which tissue samples were collected. Glycoproteins were analyzed by enzyme-linked lectin/immunosorbent assays. Gene expression was analyzed by Agilent Expression Microarray and real-time PCR using SYBR Green I dye as the intercalator. Glycosphingolipids were analyzed by HPLC using a fluorescent labeling method [1]. |
Data source location | Bioproduction Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Central 6, Tsukuba, Japan |
Data accessibility | With the article (Supplemental data) or a public repository Repository name: NCBI Gene Expression Omnibus (GEO) Data identification number: GSE115342 Direct URL to data: http://www.ncbi.nlm.nih.gov/geo/ |
Related research article | T. Okuda. A low-carbohydrate ketogenic diet promotes ganglioside synthesis via the transcriptional regulation of ganglioside metabolism-related genes. Scientific Reports. 9 (2019) 7627. https://doi.org/10.1038/s41598-019-43952-7 [1]. |