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. Author manuscript; available in PMC: 2020 Oct 1.
Published in final edited form as: Angew Chem Int Ed Engl. 2019 Aug 28;58(40):14327–14333. doi: 10.1002/anie.201907410

Figure 4.

Figure 4.

Simultaneous visualization of the deep-tissue fucosylation (Cy3 in red channel) and sialylation (Cy5 in cyan channel). a), The lateral view of zebrafish embryos co-injected with probe A and probe C at 24 hpf. b, c), The trunk (lateral view of cloche mutant (cloche −/−) or wildtype-like siblings (cloche+/−) at 48 hpf was visualized at different depth. The fluorescence in yolk was an endogenic control for the success of microinjections. d), In the cht region of Tg(c-Myb:eGFP) embryos, HSCs (green channel) do not co-localized with the labeled fucosides and sialosides. e), In Tg(c-Myb:eGFP) embryos, HSCs do not co-localize with cells expressing sLeX (red) in the cht region.