Figure 2.

Adult-specific abrogation of dBtk results in deficient footshock habituation. HIs quantifying the difference in footshock avoidance following exposure to 15 stimuli from that of same genotype naive flies are shown as the mean ± SEM for the indicated number of repetitions (n). All panels show the performance of animals expressing a dbtk RNAi-encoding transgene (btk^Ri-1/+) under the indicated driver (black bar), the driver heterozygotes (left open bars), and the RNAi-mediating transgene heterozygotes (+, right open bars). driver/+, Progeny from the cross of the w¹¹¹⁸ background driver with 36303 y¹v¹, whereas for the btk^Ri-1/+, the y¹v¹ background of btk^Ri-1 was crossed to w¹¹¹⁸ so that the two controls have equivalent backgrounds as the experimentals. Asterisks indicate significant differences from controls as detailed in the text. A, Adult limited pan-neuronal expression of btk^Ri-1 eliminates habituation to 15 footshocks. n ≥ 11 for all groups. B, Adult-limited pan-neuronal expression of an independent RNAi-mediating transgene (btk^Ri-2), also results in habituation defects. n≥9 for all groups. C, Adult-limited pan-neuronal expression of btk^Ri-1 does not yield defects in habituation to 4 min of OCT exposure. n≥9 for all groups. D, Flies expressing btk^Ri-1 under the pan-neuronal Ras2Gal4,Gal80^ts driver present adult-specific habituation defects compared with controls. n ≥ 9 for all groups. E, Adult limited btk^Ri-1 expression in glia did not precipitate deficits. n ≥ 14 for all groups. F, Pan-neuronal expression of btk^Ri-1, but at the exclusion of the MBs did not yield deficits in shock habituation. n ≥ 12 for all groups. G, dBtk is preferentially distributed within the MBs evidenced by mcD8-GFP expression under the control of the btkG4 driver (BDSC, 49182). The arrowhead indicates faint expression in the ring neurons of the ellipsoid body at the level of the pedunculi.