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. 2019 Oct 30;39(44):8730–8743. doi: 10.1523/JNEUROSCI.0633-19.2019

Table 1.

Collective avoidance indices and statistics

Genotype Mean ± SEM t Ratio p
Fig. 1C. ANOVA: F(2,58) = 1.6020, p = 0.2106
    c (control) 0.76 ± 0.03
    btkM1/+ 0.77 ± 0.05 0.106 0.9158
    btkM1/btkM1 0.66 ± 0.07 1.5444 0.1281
Fig. 1D. ANOVA: F(5,68) =0.8807, p = 0.4993
    c (control) 0.61 ± 0.02
    btkM2/M2 0.62 ± 0.04 −0.007 0.9945
    btkM1/M1 0.59 ± 0.05 0.4748 0.6365
    btkM1/ML 0.55 ± 0.05 1.0243 0.310
    btkM2/ML 0.53 ± 0.05 1.4211 0.1602
    btkML/+ 0.63 ± 0.03 −0.192 0.848
Fig. 1E. ANOVA: F(3,60) = 0.8603, p = 0.4670
    0 μm 0.53 ± 0.03
    0.1 μm 0.49 ± 0.03 1.2032 0.2339
    1 μm 0.55 ± 0.03 −0.291 0.7722
    10 μm 0.49 ± 0.04 0.9707 0.3358
Fig. 1F. ANOVA: F(3,62) = 1.9514, p = 0.1312
    c (control) 0.54 ± 0.04
    btkM1/M1 0.67 ± 0.05 −1.737 0.0877
    btkM2/M2 0.51 ± 0.06 0.3651 0.7163
    btkML/+ 0.62 ± 0.06 −1.031 0.3066
Fig. 2A. ANOVA: F(2,44) = 1.3353, p = 0.2740
    elavGAL4/+;UAS-btkRNA1/GAL80ts 0.59 ± 0.05
    elavGAL4/+;GAL80ts/+ 0.61 ± 0.04 −0.237 0.8139
    UASbtkRNAi1/+ 0.49 ± 0.07 1.3101 0.1973
Fig. 2B. ANOVA: F(2,35) = 2.0785, p = 0.1412
    elavGAL4/+;UAS-btkRNAi2/GAL80ts 0.66 ± 0.04
    elavGAL4/+;GAL80ts/+ 0.72 ± 0.03 −1.105 0.2771
    UASbtkRNAi2/+ 0.78 ± 0.03 −2.006 0.0531
Fig. 2C. ANOVA: F(2,30) =1.100 p = 0.3468
    elavGAL4/+;UAS-btkRNAi1/GAL80ts 0.73 ± 0.05
    elavGAL4/+;GAL80ts/+ 0.63 ± 0.03 1.4796 1.1502
    UASbtkRNAi-1/+ 0.68 ± 0.05 0.7939 0.4340
Fig. 2D. ANOVA: F(2,27) = 0.1719, p = 0.8431
    Ras2GAL4,GAL80ts/+; UASbtkRNAi1 0.67 ± 0.08
    Ras2GAL4, GAL80ts/+ 0.65 ± 0.05 0.1811 0.8578
    UASBTKRNAi1/+ 0.70 ± 0.04 −0.386 0.703
Fig. 2E. ANOVA: F(2,46) =2.1609, p = 0.1273
    repoGAL4,GAL80ts/UAS-btkRNAi1 0.63 ± 0.05
    repoGAL4,GAL80ts/+ 0.73 ± 0.03 −2.075 0.044
    UASbtkRNAi1/+ 0.67 ± 0.03 −0.955 0.3449
Fig. 2F. ANOVA: F(2,47) =3.2093, p = 0.0498
    elavGAL4/+;MBG80,GAL80ts/UASbtkRNAi1 0.71 ± 0.02
    elavGAL4/+; MBG80, GAL80ts/+ 0.63 ± 0.02 2.2266 0.031
    UASbtkRNAi1/+ 0.71 ± 0.03 −0.085 0.932
Fig. 3A. ANOVA: F(2,34) = 0.5313, p = 0.5929
    leoMBGAL4,GAL80ts;UAS-btkRNAi1/+ 0.46 ± 0.09
    leoMB-GAL4, GAL80ts/+ 0.56 ± 0.07 −0.905 0.3723
    UAS-btkRNAi1/+ 0.56 ± 0.07 −0.892 0.3788
Fig. 3B. ANOVA: F(5,66) = 2.7712, p = 0.0255
    leoMBGAL4,btkM1/+; GAL80ts/+ UN 0.65 ± 0.03
    leoMBGAL4,btkM1/ btkM1; GAL80ts/+ UN 0.50 ± 0.03 2.205 0.031
    leoMBGAL4,btkM1/ btkM1;GAL80ts,UASbtkS UN 0.59 ± 0.05 0.9337 0.3541
    leoMBGAL4,btkM1/+; GAL80ts/+ IN 0.56 ± 0.04
    leoMBGAL4,btkM1/ btkM1; GAL80ts/+ IN 0.45 ± 0.04 −1.265 0.211
    leoMBGAL4,btkM1/btkM1;GAL80ts,UASbtkS IN 0.62 ± 0.04 −0.914 0.364
Fig. 3C. ANOVA: F(2,39) =0.5892, p = 0.5599
    leoMBGAL4/+;UASbtkRNAi2/GAL80ts 0.65 ± 0.05
    leoMBGAL4/+;GAL80ts/+ 0.68 ± 0.03 −0.471 0.640
    UAS-btkRNAi2/+ 0.61 ± 0.06 0.6403 0.5260
Fig. 3D. ANOVA: F(2,42) =0.4747, p = 0.6256
    247MBGAL4,GAL80ts/UAS-btkRNAi1 0.63 ± 0.03
    247MBGAL4,GAL80ts/+ 0.60 ± 0.02 0.9688 0.3385
    UASbtkRNAi1/+ 0.61 ± 0.03 0.5817 0.5640
Fig. 3E. ANOVA: F(2,30) = 0.2194, p = 0.8044
    dncGAL4,GAL80ts/ UASbtkRNAi1 0.53 ± 0.02
    dncGAL4,GAL80ts/+ 0.51 ± 0.02 0.4624 0.6474
    UASbtkRNAi1/+ 0.54 ± 0.03 −0.198 0.8443
Fig. 3F. ANOVA: F(2,44) = 1.1638, p = 0.3222
    c772GAL4,GAL80ts/ UASbtkRNAi1 0.63 ± 0.03
    c772GAL4,GAL80ts/+ 0.54 ± 0.06 1.451 0.1542
    UASbtkRNAi1/+ 0.57 ± 0.03 1.1173 0.2702
Fig. 3G. ANOVA: F(2,43) = 1.891, p = 0.1766
    UASbtkRNAi-1/c739GAL4,GAL80ts 0.57 ± 0.05
    c739GAL4,GAL80ts/+ 0.46 ± 0.04 1.8657 0.0692
    UASbtkRNAi1/+ 0.53 ± 0.04 0.6998 0.488
Fig. 3H. ANOVA: F(2,47) = 3.0762, p = 0.0559
    γ lobeGAL4/+;UASbtkRNAi1/GAL80ts 0.58 ± 0.03
    γ lobe GAL4/+;GAL80ts/+ 0.49 ± 0.03 2.0924 0.0421
    UASbtkRNAi1/+ 0.59 ± 0.03 −0.238 0.8133
Fig. 3I. ANOVA: F(2,28) = 1.0786, p = 0.3548
    c305aGAL4/+;UASbtkRNAi1/GAL80ts 0.77 ± 0.03
    c305a GAL4/+;GAL80ts/+ 0.71 ± 0.02 1.3002 0.2049
    UASbtkRNAi1/+ 0.77 ± 0.04 0.0361 0.9715
Fig. 3J. ANOVA: F(2,49) = 2.5477, p = 0.0156
    VT030604GAL4/+, UASbtkRNAi1 18°C 0.74 ± 0.03
    VT030604GAL4/+, UASbtkRNAi1 25°C 0.67 ± 0.02 1.9623 0.0557
    VT030604-GAL4/+, UAS-btkRNAi1 18 > 30°C 0.78 ± 0.03 −0.972 0.3361
Fig. 3K. ANOVA: F(1,25) = 9.7589, p = 0.0046
    c305aGAL4-UAS shits UN 0.85 ± 0.03
    c305aGAL4-UAS shits IN 0.75 ± 0.01 −3.124 0.0046
Fig. 4A. ANOVA: F(5,104) = 4.7413, p = 0.0006
    control 6 sh 0.79 ± 0.04
    btkM1/M1 6 sh 0.63 ± 0.04 2.9535 0.0039
    control 10 sh 0.69 ± 0.03
    btkM1/M1 10 sh 0.60 ± 0.04 1.6587 0.1003
    control 15 sh 0.73 ± 0.02
    btkM1/M1 15 sh 0.58 ± 0.04 2.9818 0.0036
Fig. 4B. ANOVA: F(8,156) = 2.8880, p = 0.0051
    UASbtkRNAi1/elavGAL4;GAL80ts 6 sh 0.82 ± 0.02
    elavGAL4;GAL80ts/+ 6 sh 0.66 ± 0.03 3.7031 0.0003
    UAS-btkRNAi-1/+ 6 sh 0.71 ± 0.04 2.5227 0.0127
    UASbtkRNAi1/elavGAL4;GAL80ts 10 sh 0.77 ± 0.02
    elavGAL4;GAL80ts/+ 10 sh 0.66 ± 0.03 2.2533 0.0257
    UAS btkRNAi1/+ 10 sh 0.66 ± 0.06 2.0069 0.0466
    UASbtkRNAi1/elavGAL4;GAL80ts 15 sh 0.73 ± 0.04
    elavGAL4;GAL80ts/+ 15 sh 0.74 ± 0.03 −0.171 0.8644
    UASbtkRNAi-1/+ 15 sh 0.70 ± 0.03 0.6554 0.5132
Fig. 4C. ANOVA: F(2,28) = 1.2620, p = 0.2999
    0 μm 0.58 ± 0.05
    0.1 μm 0.64 ± 0.04 −0.832 0.4129
    1 μm 0.68 ± 0.04 −1.588 0.1245
Fig. 4D. ANOVA: F(2,30) = 0.0147, p = 0.9854
    Habituation,15 shocks 0.76 ± 0.02
    YO dishabituation 0.77 ± 0.02 −0.147 0.8839
    AVD after yeast puff 0.77 ± 0.02 −0.147 0.8839
Fig. 4E. ANOVA: F(2,30) = 0.0147, p = 0.9854
    btkM1/M1 habituation 6 sh 0.68 ± 0.03
    btkM1/M1 Dishabituation 6 sh 0.68 ± 0.03 0 1
    btkM1/M1 habituation 10 sh 0.73 ± 0.04
    btkM1/M1 Dishabituation 10 sh 0.73 ± 0.03 0.0617 0.9511
    btkM1/M1 AVD after yeast puff 0.73 ± 0.04 −1.102 0.2772
Fig. 5A. ANOVA: F(5,119) = 3.8885 p = 0.0027
    leoGAL4/+;UASbtkRNAi1/GAL80ts UN 6 sh 0.81 ± 0.02
    leoMBGAL4/+; UASbtkRNAi1/GAL80ts IN 6 sh 0.78 ± 0.02 1.2528 0.2128
    leoMBGAL4/+; UASbtkRNAi1/GAL80ts IN 10 sh 0.82 ± 0.01
    leoMBGAL4/+; UASbtkRNAi1/GAL80ts UN 10 sh 0.75 ± 0.02 3.053 0.0052
    leoMBGAL4/+; UASbtkRNAi1/GAL80ts IN 15 sh 0.82 ± 0.01
    leoMB-GAL4/+; UASbtkRNAi1/GAL80ts UN 15 sh 0.75 ± 0.02 3.053 0.0028
Fig. 5B. ANOVA: F(5,77) = 0.2798, p = 0.9227
    leoG4,btkM1/btkM1,G80ts,UASbtkS UN 6 sh 0.69 ± 0.03
    leoG4,btkM1/btkM1,G80ts, UASbtkS IN 6 sh 0.71 ± 0.03 −0.391 0.6966
    leoG4,btkM1/btkM1,G80ts,UAS-btkS UN 10 sh 0.69 ± 0.03
    leoG4,btkM1/btkM1,G80ts,UAS-btkS IN 10 sh 0.72 ± 0.03 −0.759 0.4505
    leoG4,btkM1/btkM1,G80ts,UAS-btkS UN 15 sh 0.69 ± 0.03
    leoG4,btkM1/btkM1,G80ts,UAS-btkS IN 15 sh 0.72 ± 0.03 −0.759 0.4505
Fig. 5C. ANOVA: F(5,77) = 1.1341, p = 0.3503
    UAS-btkRNAi1/c739-GAL4,GAL80ts UN 6 sh 0.73 ± 0.02
    UAS-btkRNAi1/c739-GAL4,GAL80ts IN 6 sh 0.72 ± 0.02 0.4393 0.6617
    UAS-btkRNAi1/c739-GAL4,GAL80ts UN 10 sh 0.74 ± 1.01
    UAS-btkRNAi1/c739-GAL4,GAL80ts IN 10 sh 0.70 ± 0.02 1.2977 0.1985
    UAS-btkRNAi1/c739-GAL4,GAL80ts UN 15 sh 0.74 ± 1.01
    UAS-btkRNAi1/c739-GAL4,GAL80ts IN 15 sh 0.70 ± 0.02 1.2977 0.1985
Fig. 5D. ANOVA: F(5,82) = 1.3274 p = 0.2614
    c305aGAL4,GAL80ts/UAS-btkRNAi1 UN 6 sh 0.78 ± 0.02
    c305aGAL4,GAL80ts/UAS-btkRNAi1 IN 6 sh 0.74 ± 0.02 1.3687 0.1751
    c305aGAL4,GAL80ts/UAS-btkRNAi1 UN 10 sh 0.78 ± 0.02
    c305aGAL4,GAL80ts/UAS-btkRNAi1 IN 10 sh 0.74 ± 0.02 1.5464 0.1261
    c305aGAL4,GAL80ts/UAS-btkRNAi1 UN 15 sh 0.78 ± 0.02
    c305aGAL4,GAL80ts/UAS-btkRNAi1 IN 15 sh 0.74 ± 0.02 1.5222 1.1321
Fig. 6A. ANOVA: F(2,28) = 1.1989, p = 0.3176
    0 μm 0.37 ± 0.04
    5 μm 0.45 ± 0.04 −1.45 0.1591
    10 μm 0.43 ± 0.03 −1.139 0.2652
Fig. 6B. ANOVA: F(2,30) = 0.8493, p = 0.4384
    0 μm 0.50 ± 0.05
    5 μm 0.54 ± 0.05 −0.624 0.5376
    10 μm 0.46 ± 0.03 0.5694 0.5736
Fig. 6C. ANOVA: F(3,44) = 1.3657, p = 0.2667
    0 μm 0.50 ± 0.03
    0.1 μm 0.57 ± 0.06 −1.432 0.1598
    1 μm 0.56 ± 0.02 −1.373 0.1772
    10 μm 0.50 ± 0.03 −0.063 0.9502
Fig. 6D. ANOVA: F(2,26) = 8.8963, p = 0.0013*
    0 μm 0.71 ± 0.03
    5 μm 0.53 ± 0.07 2.7508 0.0111
    10 μm 0.78 ± 0.03 −1.216 0.0243
Fig. 6E. ANOVA: F(2,27) = 1.3673, p = 0.2732
    c305aGAL4/+;UAS-btkRNAi1/GAL80ts 0.66 ± 0.04
    c305aGAL4/+;UAS-btkRNAi1/GAL80ts Clz 0.73 ± 0.03 −1.54 0.1361
    c305-GAL4/+;UAS-btkRNAi-1/GAL80ts Ris 0.72 ± 0.03 −1.26 0.2193
Fig. 6F. ANOVA: F(2,26) = 2.2671, p = 0.1254
    c739GAL4/+;UAS-btkRNAi1/GAL80ts 0.66 ± 0.04
    c739GAL4/+;UAS-btkRNAi1/GAL80ts Clz 0.73 ± 0.03 1.6255 0.1171
    c739GAL4/+;UAS-btkRNAi1/GAL80ts Ris 0.72 ± 0.03 −0.378 0.7085
Fig. 6G. ANOVA: F(5,95) = 1.8751, p = 0.1065
    btk M1/M1 6 sh 0.44 ± 0.05
    btk M1/M1 Clz 6 sh 0.43 ± 0.02 0.3073 0.7593
    btk M1/M1 10 sh 0.43 ± 0.05
    btk M1/M1 Clz 10 sh 0.45 ± 0.03 −0.352 0.726
    btk M1/M1 15 sh 0.20 ± 0.04
    btk M1/M1 Clz 15 sh 0.43 ± 0.03 −2.527 0.0133
Fig. 6H. ANOVA: F(5,73) = 28.6620, p < 0.0001*
    btk M1/M1 6 sh 0.35 ± 0.03
    btk M1/M1 Ris 6 sh 0.63 ± 0.03 −6.509 1.1 × 10−8
    btk M1/M1 10 sh 0.36 ± 0.02
    btk M1/M1 Ris 10 sh 0.64 ± 0.04 −6.91 2.1 × 10−9
    btk M1/M1 15 sh 0.38 ± 0.03
    btk M1/M1 Ris 15 sh 0.68 ± 0.04 −6.64 6.3 × 10−9
Fig. 6I. ANOVA: F(3,52) = 0.5727, p = 0.6356
    0 μm 6 sh 0.76 ± 0.03
    10 μm 6 sh 0.72 ± 0.03 0.952 0.3458
    0 μm 15 sh 0.78 ± 0.0.02
    10 μm 15 sh 0.73 ± 0.04 0.8233 0.4143
Fig. 6I. ANOVA: F(3,53) = 65.5164, p < 0.0001*
    0 μm 6 sh 0.78 ± 0.01
    10 μm 6 sh 0.85 ± 0.00 −10.26 7 × 10−14
    0 μm 15 sh 0.79 ± 0.01
    10 μm 15 sh 0.84 ± 0.00 −9.555 7 × 10−13

Avoidance of 45 V footshocks are shown for all genotypes per figure as indicated. Initial ANOVA: values are shown as well as individual statistical comparisons with the respective controls. Significant differences uncovered are highlighted in bold.