B20 is necessary for egestive repetition priming. A, The EN was stimulated to elicit egestive priming under control conditions (A1), and with the contralateral B20 hyperpolarized, either during the fifth (last) cycle of the motor activity (A2), or throughout all five cycles (A3). B, Filled circles represent data obtained under control conditions (no B20 hyperpolarization). Filled squares represent data obtained when B20 was hyperpolarized during the fifth cycle. Filled triangles represent data obtained when B20 was hyperpolarized during the all cycles. Hyperpolarization of B20 significantly decreased the B8 firing frequency during the protraction phase of the last cycle (n = 7). ****p < 0.0001. n.s., Not significant at p > 0.05.