Pretreatment with nimodipine can restore ghrelin-mediated feeding behavior in young (3–5 months), but not older (10 months), Tg2576 mice. A, Ghrelin (0.5 mg per kg body weight, i.p.) increased feeding in young (3–5 months) WT but not Tg2576 mice. n = 9 WT and n = 14 Tg2576 mice. Statistical analysis: two-tailed paired Student's t test (WT: t = 2.337, df = 8, p = 0.0476; Tg2576: t = 1.034, df = 13, p = 0.3201). B, Nimodipine (10 mg per kg body weight, i.p.) administered 1 h before ghrelin (0.5 mg per kg body weight, i.p.) restored the ghrelin-mediated feeding behavior in young (3–5 months) Tg2576 mice. n = 12 WT and n = 12 Tg2576 mice. Statistical analysis: two-tailed paired Student's t test (WT: t = 3.986, df = 11, p = 0.0021; Tg2576: t = 4.284, df = 11, p = 0.0013). C, Nimodipine (10 mg per kg body weight, i.p.) administered 1 h before ghrelin (0.5 mg per kg body weight, i.p.) failed to restore the ghrelin-mediated feeding behavior in older (10 months) Tg2576 mice. n = 12 WT and n = 14 Tg2576 mice. Statistical analysis: two-tailed paired Student's t test (WT: t = 4.128, df = 11, p = 0.0017; Tg2576: t = 1.754, df = 13, p = 0.103). Data are expressed as individual mice. *p < 0.05; **p < 0.01; paired t test. n.s. = not significant.