Table 5.
Properties of VSD imaging signals to parasubicular stimulationa
| PaS | Signal size | Latency (ms) | Decay time (ms) | Difference between layers per age group | |||
|---|---|---|---|---|---|---|---|
| P9–P11 | LII | 4357 ± 382 | 5.0 ± 0.3 | 127 ± 56 | D: ANOVA df = 3, p = 0.395 | L: KW 5.20, df = 3, p = 0.004 | S: ANOVA df = 3, F = 41, p = 0 0.000 |
| (N = 29) | (N = 27) | (N = 10) | |||||
| LIII | 2936 ± 282 | 4.6 ± 0.4 | 96 ± 31 | ||||
| (N = 29) | (N = 27) | (N = 10) | |||||
| **p = 0.000 | |||||||
| LV | 1661 ± 420 | 4.6 ± 0.3 | 110 ± 62 | ||||
| (N = 29) | (N = 8) | (N = 5) | |||||
| **p = 0.000 | |||||||
| *p = 0.000 | |||||||
| LVI | 1141 ± 301 | 8.2 ± 0.9 | 101 ± 31 | ||||
| (N = 29) | (N = 8) | (N = 5) | |||||
| **p = 0.000 | **p = 0.000 | ||||||
| *p = 0.000 | *p = 0.000 | ||||||
| #p = 0.002 | |||||||
| P12–P14 | LII | 5036 ± 312 | 4.1 ± 0.2 | 82 ± 10, (N = 25) | D: KW 8.37, df = 3, p = 0.39 | L: ANOVA df = 3, F = 6.03, p = 0.000 | S: ANOVA df = 3, F = 8.79, p = 0.000 |
| (N = 32) | (N = 32) | ||||||
| LIII | 4224 ± 294 | 3.6 ± 0.3 | 138 ± 19 | ||||
| (N = 32) | (N = 32) | (N = 22) | |||||
| **p = 0.042 | **p = 0.020 | ||||||
| LV | 5500 ± 442 | 4.2 ± 0.2, | 111 ± 10 | ||||
| (N = 32) | (N = 32) | (N = 26) | |||||
| *p = 0.001 | **p = 0.015 | ||||||
| #p = 0.000 | |||||||
| LVI | 3639 ± 327 | 5.2 ± 0.2 | 96 ± 32 | ||||
| (N = 32) | (N = 32) | (N = 16) | |||||
| **p = 0.000 | **p = 0.007 | **p = 0.058 | |||||
| #p = 0.000 | *p = 0.000 | ||||||
| #p = 0.011 | |||||||
| P15–P17 | LII | 3361 ± 174 | 3.9 ± 0.3 | 40 ± 2 | D: KW 26.9, df = 3, p = 0.000 | L: ANOVA df = 3, F = 4.36, p = 0.006 | S: ANOVA df = 3, F = 21.2, p = 0.000 |
| (N = 29) | (N = 26) | (N = 26) | |||||
| LIII | 3021 ± 164 | 4.0 ± 0.2 | 80 ± 7 | ||||
| (N = 29) | (N = 26) | (N = 26) | |||||
| **p = 0.000 | |||||||
| LV | 4593 ± 269 | 3.6 ± 0.3 | 64 ± 4 | ||||
| (N = 29) | (N = 26) | (N = 26) | |||||
| **p = 0.000 | **p = 0.000 | ||||||
| *p = 0.000 | *p = 0.033 | ||||||
| LVI | 3061 ± 219 | 5.1 ± 0.3 | 63 ± 5 | ||||
| (N = 29) | (N = 26) | (N = 26) | |||||
| #p = 0.000 | **p = 0.008 | **p = 0.001 | |||||
| *p = 0.017 | |||||||
| #p = 0.001 | |||||||
| P28–P30 | LII | 1890 ± 199 | 5.3 ± 0.4 | 24 ± 4 | D: KW 12.8, df = 3, p = 0.005 | L: ANOVA df = 3, F = 1.5, p = 0.218 | S: ANOVA df = 3, F = 6.3, p = 0.001 |
| (N = 19) | (N = 14) | (N = 12) | |||||
| LIII | 1609 ± 155, | 4.2 ± 0.3, | 59 ± 8 | ||||
| (N = 19) | (N = 14) | (N = 11) | |||||
| **p = 0.003 | |||||||
| LV | 2063 ± 201 | 4.2 ± 0.4 | 65 ± 9 | ||||
| (N = 19) | (N = 14) | (N = 10) | |||||
| *p = 0.004 | **p = 0.000 | ||||||
| LVI | 1467 ± 172 | 5.0 ± 0.5 | 52 ± 8 | ||||
| (N = 19) | (N = 14) | (N = 10) | |||||
| **p = 0.007 | **p = 0.017 | ||||||
| #p = 0.000 | |||||||
| P59–P61 | LII | 2459 ± 507 | 4.1 ± 0.6 | 34 ± 5 | D: KW 10.6, df = 3, p = 0.014 | L: ANOVA df = 3, F = 0.6, p = 0.603 | S: ANOVA df = 3, F = 3.83, p = 0.024 |
| (N = 8) | (N = 8) | (N = 8) | |||||
| LIII | 2128 ± 362 | 4.5 ± 0.8 | 78 ± 12 | ||||
| (N = 8) | (N = 8) | (N = 8) | |||||
| **p = 0.006 | |||||||
| LV | 2355 ± 395 | 4.8 ± 1.2 | 42 ± 8 | ||||
| (N = 8) | (N = 8) | (N = 5) | |||||
| LVI | 1613 ± 273 | 5.3 ± 0.4 | 57 ± 6 | ||||
| (N = 8) | (N = 8) | (N = 5) | |||||
| **p = 0.005 | **p = 0.030 | ||||||
| #p = 0.012 | |||||||
aThe rows of the table represent five different age groups (P9–P11, P12–P14, P15–P17, P28–P30, and P59–P61). The last column presents the results of the statistical test used to assess the differences between layers. Homogeneity was tested with Levene's test. If assumptions for parametric tests were not met, Kruskal–Wallis (KW) followed by Mann–Whitney U tests were performed instead. N indicates the number of VSD responses analyzed. The rows represent average parameters of each layers of all age groups ± SEM. S, Signal size (the integrals of the optical signal curves, which indicate the sum of membrane potential changes); L, latency (measured from the beginning of the stimulus artifact to the start of the fluorescent change response); D, decay time (calculated by fitting a curve from 25 ms up to 400 ms after the last stimulus to the negative slope).
**Significantly different from LII.
*Significantly different from LIII.
#Significantly different from LV.