Fig. 2.

PRDX3 acetylation plays a vital role in intestinal I/R injury.

(A) PRDX3 acetylation in the intestine subjected to 45 min of intestinal ischemia followed by 1–8 h of reperfusion, n = 6. (B) PRDX3 acetylation in Caco-2 cells after reoxygenation for 1–8 h, n = 6. (C) PRDX3 acetylation in Caco-2 cells after treatment with NAM for 2–8 h, n = 6. (D) PRDX3 acetylation in Caco-2 cells after treatment with TSA for 2–8 h, n = 6. (E–G) Caco-2 cells were pretreated with NAM and/or transfected with the PRDX3 expression plasmid and then subjected to H/R. (E) Mitochondrial H₂O₂ level, n = 8. (F) MitoSOX Red staining and flow cytometry analysis of cells stained with MitoSOX dyes. Scale bar = 25 μm, n = 6. (G) Representative immunoblot of cleaved caspase-3 in Caco-2 cells, n = 3. *p < 0.05, **p < 0.01. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)