Expression of SIRT3 and PRDX3 in the ischemic intestine of clinical patients.
(A) The expression of PRDX3 protein was normalized to β-actin expression in tissue lysates from the ischemic intestines of clinical patients, n = 3. (B) SIRT3 protein expression was normalized to β-actin expression in tissue lysates from the ischemic intestines of clinical patients, n = 3. (C) CoIP of PRDX3 with SIRT3. The input represents the total protein extract used in IP. IB, immunoblotting; IP, immunoprecipitation, n = 3. (D) PRDX3 acetylation, n = 3. *p < 0.05, **p < 0.01.