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. 2019 Oct 29;9:15457. doi: 10.1038/s41598-019-51893-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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3D TPM, CFM and CRM images of an ex-vivo mouse conjunctiva tissue. (a–c) moxifloxacin-based TPM and CFM images, and an CRM image of the same mouse conjunctiva tissue respectively. Three en-face images at different depths of the near surface, 15 μm and 35 μm deep from the surface are presented. The first two superficial images showed the epithelium and the last one image showed the substantia propria. Green color in both TPM and CFM images represented moxifloxacin fluorescence and blue color in the TPM image represented SHG. White and yellow arrows marked goblet cell clusters and blood vessels, respectively.