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. 2019 Oct 29;9:15532. doi: 10.1038/s41598-019-52069-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TPM images of gatifloxacin instilled in vivo mouse corneas without (a–c and j) and with (d–f and k) ultrasound treatment. TPM images were acquired after 10-minute gatifloxacin incubation. (a–c) and (d–f) En-face TPM images of the mouse corneas at 3 different depths without and with ultrasound treatment, respectively (Supplementary Video 3, 4). En-face TPM images at 3 different layers of the superficial epithelium (first column), middle epithelium (second column), and basal epithelium (third column) are presented. (g–i) En-face auto-fluorescence (AF) TPM images of the mouse cornea. (j,k) Cross-sectional TPM images of the gatifloxacin instilled mouse corneas without and with ultrasound, respectively. (l) A cross-sectional AF TPM images of the mouse cornea. The scale bar indicates 100 µm.