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. 2019 Oct 29;9:15516. doi: 10.1038/s41598-019-51332-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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LOFU modulates the expression and cellular distribution of Hsp gene family members in 4T1 breast cancer cells. (A) LOFU augments the expression of Hsp RNA. qRT-PCR was performed on cell lysates, isolated 4 hours after treatment of 4T1 cells, (ANOVA Hsp70 p = 0.05, Hspb1 p = 0.05). Figure is representative of 3 independent experiments. (B) LOFU increases cellular HSP70 protein concentration. HSP70 ELISA was performed with total cell lysate, obtained 4 hours and 24 hours after LOFU treatment. (C) LOFU induces translocation of cytosolic HSP proteins to the cell surface. Flow cytometric analysis of cell surface expression of HSP70 and HSP90 was performed 4 hours after LOFU treatment (ANOVA HSP70 p = 0.02, HSP90 p = 0.01). (D) LOFU increase HSP70 protein secretion. HSP70 protein concentration in the cell culture medium, obtained 4 hours and 24 hours after LOFU treatment was measured by ELISA.