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. 2019 Oct 29;9:15516. doi: 10.1038/s41598-019-51332-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Combination Treatment with LOFU + RT protects mouse from tumor re-challenge. (A–C) Prior treatment with LOFU + RT inhibits secondary tumor growth. 5 naïve mice (A), 4 WT mice (B), and 8 PSA-Tg mice with primary tumors cured (C) were injected with TPSA23 cells and tumor volume was followed up to 60 days post inoculation. Naïve mice are wild-type C57BL/6 mice that have never been injected with TPSA23 prior to this experiment. (D) Combination LOFU + RT reduces average secondary tumor volume on day 56. Average re-challenge tumor volume with standard deviation (Kruskal-Wallis test p = 0.0056) One out of four WT mice and two out of eight PSA-transgenic mice showed complete tumor growth inhibition.