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. 2019 Oct 29;9:15495. doi: 10.1038/s41598-019-51751-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Knock down transfection of SOX17 in ECC-1 cells and inhibition using a chemical inhibitor results in decreased adhesion of ‘blastocyst-mimic’ spheroids. Knockdown transfection of ECC-1 cells with a CRISPR/Cas9 SOX17 knock-down plasmid mediated a significant reduction in spheroid adhesion across all knock-down cell clones (A, KD1-5) when compared to untransfected control ECC-1 cells (A, E/P) and ECC-1 cells transfected with a control plasmid (A, CP,****p < 0.0001). Inhibition of the group F Sox proteins using 20 μM MCC177 (B, Sox F inhibitor) resulted in a significant decrease in spheroid adhesion (****p < 0.0001), while 50 μM MCC177 completely inhibited spheroid adhesion (****p < 0.0001). Data presented as mean ± SEM, n = 5.