Fig. 2.

MCH neurons communicate to glutamatergic neurons in the CA1v. a (left) A representative injection site for fluorogold in the CA1 region of the vHP; scale = 200 µm. (right) Fluorogold (pseudocolored blue) colocalizes with MCH immunofluorescence (red) in a region of the lateral hypothalamic area (white arrows indicate colocalization); scale = 100 µm. b Fluorescence in situ hybridization for MCHR1 mRNA (green) and the glutamatergic marker vGLUT1 (red), with DAPI counterstain (blue). c Fluorescence in situ hybridization for MCHR1 mRNA (green) and the GABAergic marker GAD2 (red), with DAPI counterstain (blue); scale = 50 µm