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. 2019 Oct 23;10:988. doi: 10.3389/fgene.2019.00988

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Copyright © 2019 Li, Hu, Wang, Deng, Yang, Hu, Li and Wang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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mRNA and miRNA transcriptome sequencing and identification of differentially expressed (DE) mRNAs and miRNAs in either granulosa or theca layers. (A, B): Principal component analysis of the mRNA and miRNA transcriptomes among 18 libraries. (C, D) Pearson correlation analysis of the mRNA and miRNA transcriptome among nine granulosa or theca layers. (E, F) Number of DE mRNAs and miRNAs in either granulosa or theca layers. (G–J) Heatmap analysis of DE mRNAs (G, H) and miRNAs (I, J) in granulosa or theca layers. 4–6: follicles with 4–6 mm in diameter; 8–10: follicles with 8–10 mm in diameter; F5: hierarchical F5 follicle. GP4_1, GP4_2, and GP4_3 represent three replicates of GP4, and similar definitions have been used for GP8, GHF5, TP4, TP8, and THF5.