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. 2019 Oct 29;9:15507. doi: 10.1038/s41598-019-51947-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of intracellular MIF on the overall growth of HapT1 cells in-vitro. (A) Immunoblot analysis showing the MIF expressed by the HapT1 cells (Lysates of two different passages). (B) Graph showing the reduced expression of MIF after 48 hours of siRNA transfection (n = 3). Each bar represents the mean ± the SEM. ***p < 0.0001 using a Student t-test. (C) Graph showing the percentage of viable cells upon siRNA transfection (n = 3). Each bar represents the mean ± the SEM. **p < 0.001 using a Student t-test. (D) Graph showing the percentage of viable cells upon treating with MaMIF (100 ng/ml), ISO-1 (100 µg/ml) and MaMIF + ISO-1 for 48 hours (n = 3). Data represents the mean ± SEM, *p < 0.05; **p < 0.001; ***p < 0.0001 using one-way ANOVA with Bonferroni’s Multiple Comparison Test.