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. 2019 Oct 29;9:15565. doi: 10.1038/s41598-019-52129-1

Figure 4.

Figure 4

The contractility of cell pairs increased after their fusion and raise on elongated micropatterns. (A) Epifluorescence images of C2C12 cell pairs unfused (D) and fused (FD) on circular and rectangular (1:7 aspect ratio) FN micropatterns and stained for mitochondria with Mito Tracker. Scale bars are 20 µm. (B) Representative maps of the contractile stress exerted by C2C12 cell pairs fused on circular and rectangular FN micropatterns. (C) Evolution of the total stress for unfused (D), (plain bars) and fused (FD, hashed bars) C2C12 cell pairs on circular (in grey) and rectangular (in purple) FN micropatterns. Circle D: n = 8; Circle FD: n = 5; rectangle D: n = 6 and rectangle FD: n = 8. **p < 0.01.