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. 2019 Oct 29;9:15566. doi: 10.1038/s41598-019-52208-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phenotypic characterization of OPN-expressing cells in small airways during COPD (GOLD stage II). Immunofluorescence was used to detect possible co-localization of OPN and mucin 5AC (MUC5AC) in goblet cells (A), uteroglobin (UTG) in club cells (B), p63 in basal cells (C), and forkhead box protein J1 (FOXJ1) in ciliated cells (D). In the overlay, a high degree of co-localization was observed for OPN and MUC5AC in goblet cells and UTG in club cells. Scale bars: A = 50 µm, B–D = 10 µm.