Table 1.

Thermal stability of D3-L11 determined by DSC.

	TM (°C)a	ΔTM (°C) (TMmutant − TMWT)	Order of stability (WT, reference)
WT	66.79 ± 0.01	0	0
E32A	67.26 ± 0.02	0.47	+1
Y52A	68.79 ± 0.01	2.00	+4
H54A	66.78 ± 0.01	−0.01	−1
T55A	63.74 ± 0.01	−3.05	−4
K101A	62.43 ± 0.02	−4.36	−7
Y102A	66.38 ± 0.01	−0.41	−2
P104A	63.54 ± 0.02	−3.25	−5
R106A	68.04 ± 0.01	1.25	+3
F107A	66.24 ± 0.01	−0.55	−3
S113A	67.59 ± 0.01	0.80	+2
D115A	62.92 ± 0.02	−3.87	−6

^aValues ± S.E. of the curve fitting.