. 2019 Oct 29;9:15481. doi: 10.1038/s41598-019-50722-y

Table 3.

Thermodynamic binding parameters (SPR)^a.

	ΔG_vH	ΔΔG_vH^b	ΔH_vH	ΔΔH_vH^b	−TΔS_vH	−TΔΔS_vH^b	ΔC_p,vH	ΔG^‡	ΔH^‡	−TΔS^‡
	kcal mol⁻¹	kcal mol⁻¹	kcal mol⁻¹	kcal mol⁻¹	kcal mol⁻¹	kcal mol⁻¹	kcal K⁻¹ mol⁻¹	kcal mol⁻¹	kcal mol⁻¹	kcal mol⁻¹
WT	−14 ± 2	—	−17 ± 1		3.1 ± 1.1	—	−0.024 ± 0.186	8.0 ± 0.9	8.4 ± 0.6	−0.32 ± 0.66
Y52A	−10 ± 1	4.0 ± 2.2	−11 ± 1	6.0 ± 1.4	0.68 ± 0.54	−2.4 ± 1.2	−0.16 ± 0.07	8.8 ± 0.2	4.3 ± 0.1	−4.5 ± 0.1
Y102A	−8.7 ± 4.0	5.3 ± 4.5	−12 ± 3	5.0 ± 3.2	3.2 ± 2.9	0.1 ± 3.1	0.15 ± 0.48	8.8 ± 1.3	6.7 ± 0.9	−2.1 ± 0.9

^aValues ± S.E of the curve fitting based on van’t Hoff or Eyring plots.

^bΔΔG_vH = ΔG_vH,mutant − ΔG_vH,WT, ΔΔH_vH = ΔH_{vH, mutant} − ΔH_{vH, WT}; −TΔΔS_vH = (−TΔS_{vH, mutant}) − (−TΔS_vH,WT).