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. 2019 Oct 29;6:238. doi: 10.1038/s41597-019-0253-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Quality metrics of raw and aligned reads. (a) The distribution of median phred quality score per base across all samples in fetal RNA-seq, adult RNA-seq, fetal RRBS, and adult RRBS data sets. (b) Number of reads before and after alignment of all samples in four data sets. (c) Distribution of percentages of aligned reads across different mapping quality ranges in four data sets. (d–e) An example of percentage of sequence content per base before and after NuGEN diversity trimming in two RRBS data sets (sample: C1_6).