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. 2019 Oct 29;9:15554. doi: 10.1038/s41598-019-52171-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Induction of the secretory volume decrease (SVD) in human colonic epithelial Caco-2 cells by four herbal components in Japanese Kampo laxative medicines Junchoto and Mashiningan. (a) Time courses of changes in the mean cell volume. At time 0, Kyonin (KYN), Tonin (TON), Masinin (MSN), or Daio (DIO) was applied at 400 μg/ml except under the control condition. SVD events were monitored by the electronic sizing technique. (b) Percentage of the cell volume after 30 min of treatment with each herbal component relative to the initial cell volume. Each column represents the mean ± S.E.M. (n = 5). *P < 0.05 compared with the control volume.