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. 2019 Oct 29;9:15469. doi: 10.1038/s41598-019-51939-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TonEBP target gene expression under hypoosmotic conditions is unaffected by inhibition of primary cilia formation in NP cells. (a–f) mRNA levels of AR/Akr1b1, SMIT/Slc5a3 and TauT/Slc6a6 under hypoosmotic conditions (200 mOsm/kg H₂O) following knockdown of Ift88 (a–c) or Kif3a (d–f). Stable silencing of either Ift88 or Kif3a does not affect the trend of downregulation in SMIT/Slc5a3 and TauT/Slc6a6 under hypoosmotic conditions (n ≥ 3). Data are represented as scatter plots (mean ± SEM). ns = not significant. One-way ANOVA or Kruskal-Wallis test with Sidak’s or Dunn’s multiple comparison test was used based on the distribution of the data to determine statistical significance.