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. 2019 Oct 29;10:4911. doi: 10.1038/s41467-019-12839-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Knockdown of Foxg does not affect the atrial siphon primordia. Z-projected images of the trunk region of a a control larva and b a larva developed from an egg injected with the Foxg MO, which were stained with phalloidin. Arrowheads indicates the anterior tip. Note that the palps were lost in the Foxg morphant larva. Atrial siphon primordia in the right side are shown in higher magnification views. The openings are indicated by arrows. In higher magnification views, nuclei are stained by DAPI are shown in gray