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. 2019 Oct 29;10:4911. doi: 10.1038/s41467-019-12839-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — Efna.d is responsible for restricting Foxg expression in the ANB. a Double in situ hybridization of Efna.d (magenta) and Foxg (green). b Depiction for Efna.d expression in the ANB. c Immunostaining of dpERK (magenta) in an embryo in which ANB cells were marked with Foxc > Gfp. The middle rows show ectopic signals for dpERK (arrowheads). d Depiction of ANB cells stained with the anti-dpERK antibody in neurula embryos introduced with Foxc > dn-Eph.c. e–g In situ hybridization of e Foxg and f, g Emx in e, g neurula embryos introduced with Foxc > dn-Eph.c and f an unperturbed neurula embryo. In (e), the middle two rows of cells expressed Foxg ectopically (compared with Fig. 1b). In (f) and (g), while Emx expression in the tail was not changed, it was lost in the ANB of a Foxg morphant (arrowhead). The number of embryos examined and the proportion of embryos that each panel represents are shown within the panels. a, c, e Anterior views in which the ventral side is up. f, g Dorsal views in which the anterior is up. h Lateral view showing the morphology of a larva introduced with Foxc > dn-Eph.c. ANB cells were marked with GFP by cointroduction of the Foxc > Gfp construct and counterstained with phalloidin (gray)