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. 2019 Oct 29;9:15458. doi: 10.1038/s41598-019-51865-8

Figure 3.

Figure 3

Glioma cells rely on LXRβ signaling to maintain cholesterol, cell viability and cell proliferation. (A) Fold change in cholesterol levels in glioma TS600 LXRβ CRISPR knockout cells (crLXRb-1) and non-targeting control (crNT). For each knockout condition, levels were normalized to sparse. Error bars indicate SEM for at least 3 experiments. *p < 0.05 for paired t-test of sparse vs. dense. (B) BrdU cell proliferation assay in TS600 crNT and crLXRb-1 cells. BrdU quantitation is normalized to sparse for each knockout condition. Error bars indicate SEM for at least 3 replicates. ***p = 0.0008 for two-tailed unpaired t-test. (C) Cell death in TS600 crNT and crLXRb-1 cells plated at increasing cell numbers, as measured with CellTox Green. Error bars indicate SD for at least 4 replicates. Data shown are representative of at least 3 biological replicates.