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. 2019 Oct 29;9:15458. doi: 10.1038/s41598-019-51865-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ABCA1 expression is not essential for overall cholesterol levels, cell proliferation, or cell viability. (A) Fold change in cholesterol levels in glioma TS600 ABCA1 CRISPR knockout cells (crABCA1-1 and crABCA1-3) and non-targeting control (crNT). For each knockout condition, levels were normalized to sparse. Error bars indicate SEM for at least 3 experiments. (B) BrdU cell proliferation assay in TS600 crNT and crABCA1 cells. BrdU quantitation is normalized to sparse for each knockout condition. Error bars indicate SEM for at least 3 replicates. ***p = 0.0008, **p = 0.005, *p = 0.03 for two-tailed unpaired t-test. (C) Cell death in TS600 crNT and crABCA1 cells plated at increasing cell numbers, as measured with CellTox Green. Error bars indicate SD for at least 4 replicates. Data shown are representative of at least 3 biological replicates.